Fig. 3
From: The activity of TRAF RING homo- and heterodimers is regulated by zinc finger 1
TRAF6 dimers with a single conjugate binding site are active. a Single-turnover assay using fluorescently labelled conjugate performed as in Fig. 2c. Mutant proteins that disrupted either the E2 interface (IL/DR) or ubiquitin binding (ArgZF1) were incubated with the conjugate alone, or following mixing (far right). Schematics indicate the site of each mutation on TRAF6. b The formation of diubiquitin from panel a was quantified. Experiments were performed in duplicate; error bars represent range of measurements. c Coomassie blue stained chain-building assay using TRAF6 RZ3 mutants from panel a
