Fig. 3

Reduced ZIKV burden in Ifnar1 ^−/− mice adoptively transferred with DENV2-exposed CD8⁺ T cells. Naive (1 × 10⁷) and DENV2-exposed CD8⁺ T cells (1 × 10⁷) from Ifnar1 ^−/− mice were adoptively transferred to naive recipient Ifnar1 ^−/− mice. Three days post ZIKV challenge (1 × 10⁴ FFU of ZIKV FSS13025, retro-orbital route), cross-reactive peptide-specific CD8⁺ T cell responses (a) and ZIKV levels in sera and indicated organs (b) were detected by ICS assay and FFA, respectively. Data are pooled from two independent experiments (n = 3–4 mice per group per experiment) and expressed as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001,****p < 0.0001. a A Kruskal–Wallis one-way ANOVA. b Two-tailed Mann–Whitney test. Supplementary Tables 1 and 2 provide exact values of n and p