Fig. 6

Major SA antagonistic pathways are not responsible for enhanced susceptibility to Pst DC3000 at elevated temperature. a ET, c JA and e ABA marker gene expression in plants (n = 3) 24 h after vacuum-infiltration with mock or Pst DC3000. Bacterial growth in WT and b ein3 eil1, d myc2/3/4, f aba2 and g camta2/3 mutant plants (n = 4) 3 days after vacuum-infiltration with Pst DC3000. h SA metabolite quantification and i SA marker gene expression in WT and camta2/3 mutant plants (n = 4) 3 days after vacuum-infiltration with mock or Pst DC3000. Gene expression and LCMS data were processed and analysed as described in Fig. 2. Data are presented as the mean ± s.e.m. with n = biological replicates and are representative of three independent experiments. Letters indicate statistical significance based on a two-factor ANOVA with Tukey’s HSD post hoc analysis (p < 0.05); samples sharing letters are not significantly different. Data in h and i were analysed in two groups based on temperature