Fig. 6
Type I interferons suppress inflammasome activation in WAS. a WT or WAS KO BMDCs were stimulated with type-1 IFNs or IFN-γ (500 U/ml) for 16 h. Cells were then primed with LPS (100 ng/ml) for 3 h, followed by stimulation with mM ATP for 30 min (left); 10 μM nigericin for 1 h (middle) or WT EPEC (MOI of 5, right) for 3 h. IL-1β protein levels were quantified by ELISA. Cytokine levels from three independent experiments performed in duplicate are shown as mean protein concentration ± SEM. Two-sided Student’s t-test. b PBMCs from healthy controls (n = 3) or WAS patients (n = 3) were stimulated with IFN-α, IFN-β or IFN-γ for 16 h, followed by LPS (100 ng/ml) for 3 h and then followed by ATP (3 mM) stimulation for 30 min. Culture supernatants were subjected to IL-1β quantification by ELISA. Results are presented as mean protein concentration ± SEM from three independent experiments performed in duplicate. Two-sided Student’s t-test. *p < 0.05; **p < 0.01; ***p < 0.001; NS = not significant
