Fig. 2
From: Multiplex single-cell visualization of nucleic acids and protein during HIV infection
Time course of vRNA detection. a TZM-bl cells were infected with HIV-1 at an MOI of 0.2. Cells were then fixed and stained at the indicated times. Cells were stained for vRNA (PS-1; green) and nuclei (blue). At 12 hpi, ~15% of infected cells exhibited a transcription burst (12 h, i) whereas most cells did not yet produce nascent vRNA (12 h, ii). Scale bars represent 10 µm. b Foci of vRNA were counted in 300–340 cells per time point, using Gen5 software. The average number vRNA foci per cell was calculated and shown in the graph with standard deviation indicated (n = 2 independent experiments)
