Fig. 1
From: Receptor-binding loops in alphacoronavirus adaptation and evolution
Characterization of soluble fragments of the HCoV-229E S-protein and hAPN. a HCoV-229E infection of L-132 cells in the presence of: PBS, the HCoV-229E S1 domain (residues 17–560 at 10 µM), and the HCoV-229E RBD (residues 293–435 at 30 µM). Statistics were obtained from three independent experiments. Statistical significance (ANOVA): ***p < 0.001; error bars correspond to the standard deviation. b Representative images of HCoV-229E infection of L-132 cells in the presence of the hAPN ectodomain at various concentrations. Green fluorescence measures the expression of the viral S-protein. Magnification (100×) and scale bar = 20 µm. c Quantitation of the hAPN inhibition experiment. Statistics were obtained from three independent experiments. Statistical significance (ANOVA): ***p < 0.001
