Fig. 8
From: Primer synthesis by a eukaryotic-like archaeal primase is independent of its Fe-S cluster
Primer synthesis assay for the cross-linked Chimera. For all experiments, wild-type Chimera, PriS E34C and PriX K110C single mutants and PriS E34C, PriX K110C double mutants were analyzed. Cross-linking reactions were analyzed at 20′ and 40′ time points, with and without BMOE. The reaction products were separated by denaturing PAGE, stained with SYBR Gold and detected by phosphorimaging. a Primer synthesis assay on a mixed-sequence ssDNA 30mer template and NTPs. b Primer synthesis assay on a mixed-sequence ssDNA 38mer template, RNA 18mer primer and NTPs. c Primer synthesis assay on a mixed-sequence ssDNA 38mer template, RNA 18mer primer and dNTPs
