Fig. 4

Heme induces a conformational switch in DGCR8 that facilitates RNA binding. a, b Representative negative stain electron micrographs of a MP^Heme and b MP^C352S after crosslinking with glutaraldehyde, with zoomed-in view of the selected particles shown underneath. c Thermal denaturation of DGCR8 in heme-saturated vs. heme-deficient states shows that the melting temperature (T_m) increases by 1.5 °C in the presence of heme. d EMSA with 5′ end-labeled pre-miR-143 shows that DGCR8^Heme binds with an increased affinity compared to that of the DGCR8^C352S mutant. “−” lane contains no protein. Protein concentrations are (left to right): 0.13, 0.26, 0.52, 1.04, and 2.08 μM