Table 1 Results of SV-AUC and SEC-MALS experiments
From: Heme enables proper positioning of Drosha and DGCR8 on primary microRNAs
Predicted mass for monomeric DGCR8 (kDa) | Predicted mass for dimeric DGCR8 (kDa) | svAUC | SEC-MALS | DGCR8 state | |||||
|---|---|---|---|---|---|---|---|---|---|
Sed. coeff. (S) | Molar mass (kDa) | Frictional ratio | RMSD | Molar mass by RI (kDa) | Zimm R2 | ||||
DGCR8Heme | 61.1 | 121.5 | 4.39 | 119 | 1.73 | 0.0049 | ND | ND | Dimer |
DGCR8C352S | 59.8 | 120.9 | 4.05 | 120 | 1.94 | 0.0069 | 121.6 | 0.9894 | Dimer |
DGCR8Apo | 60.5 | 120.9 | ND | ND | ND | ND | 125.3 | 0.9995 | Dimer |
MPHeme | 179.4 | 240.5 | 7.50 | 230 | 1.59 | 0.0054 | ND | ND | Dimer |
MPC352S | 179.4 | 239.8 | 7.31 | 215 | 1.57 | 0.0053 | 252.7 | 0.9962 | Dimer |
MPΔHBR | 149.2 | 178.5 | ND | ND | ND | ND | 151.5 | 0.9125 | Monomer |
