Fig. 4
From: Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae
Quantitative analysis of teichoic acids and choline-binding proteins. Bacteria were grown in THY medium to A 600 = 0.35–0.45 (for LytA 0.4 and 0.8), washed and incubated with antibodies. a The amount of P-Cho and the Forssman antigen of teichoic acids were determined using specific primary antibodies (TEPC-15, anti-Forssman) and secondary Alexa488-labeled antibody in a flow cytometry-based assay. b Surface-associated CBPs were analyzed using specific polyclonal mice IgG against the individual CBPs and secondary Alexa488-labeled antibodies in a flow cytometry-based assay (see also Supplementary Fig. 9 for representative histograms). The values are represented as the geometrical mean fluorescence intensity multiplied with percent-gated events. Results are expressed as means ± s.d. (each experiment was performed at least three times). *p < 0.05; ns not significant (Student's unpaired t test)
