Fig. 6
Transcriptome of siNOTCH1 indicates changes in cell cycle, inflammatory genes, and regulators of calcium. a Cultures of HAECs treated with siNOTCH1 and siControl were analyzed for transcriptional changes by microarray. Heatmap to display differentially expressed genes (≥0.5 (log2) upregulated or ≤ −0.5 (log2) downregulated with P < 0.001) marking NOTCH1 target genes and upregulated cell cycle, inflammatory genes and regulators of intracellular calcium. (b–d) Volcano plot visualization of the differentially expressed genes between siNOTCH1 and siControl. Colors indicate as follows: gray—no differential expression, black—genes whose fold change is either ≤0.50 or ≥1.5, red—genes known to contribute to the regulation of cell cycle, orange—genes involved in inflammation, and green—genes known to be regulators of intracellular calcium. (e, f) Confocal images of HAECs exposed to flow for 48 h in the presence of γ-secretase inhibitor DAPT or vehicle and stained for F-actin, VECAD, and NFAT2. Note the presence of nuclear NFAT2 and gaps between cells only in DAPT-treated monolayers (marked by white arrows). Scale bars = 20 µm
