Table 1 Crystallographic data
Crystal | EtfAB/Bcd |
|---|---|
Crystallization conditions | 0.1 M calcium acetate; 14 % (v/v) PEG 400; 0.1 M MES, pH 6.5 |
Cryo conditions | 0.1 M calcium acetate; 0.1 M MES, pH 6.5; 30 % PEG 400; 10 µM FAD |
Data collection | |
Space group | I4122 |
Wavelength [Å] | 1.0 |
Resolution range [Å] | 50.0-3.1 (3.2-3.1) |
Unit cell a; b; c [Å] | 177.1, 177.1, 493.2 |
Redundancy | 7.2 (7.6) |
Completeness [%] | 98.9 (98.4) |
Rsym [%] | 14.3 (364.1) |
I/σ(I) | 11.1 (0.9) |
Refinement | |
No. reflections | 69810 (6711) |
Mol. asym. unit | 2 EtfAB/Bcd |
No. of atoms: polypeptide, ligands | 14744, 421 |
R work, R free (%) | 20.8 (47.5), 25.9 (51.9) |
B average (Å2): polypeptide, ligands | 130.3, 150.8 |
R.m.s. deviation | |
Bond lengths (Å) | 0.018 |
Bond angles (°) | 1.87 |
Ramachandran plot favored, outliers (%) | 92.0, 0.4 |
PDB ID | 5OL2 |
