Table 1 Transcripts and strains in this study
Name | Description | Ref |
|---|---|---|
Transcripts | ||
Mr-OPY2-S | The short transcript of the Mr-OPY2 gene | This study |
5′ UTR S | The 5’UTR of the short transcript Mr-OPY2-S | |
Mr-OPY2-L | The long transcript of the Mr-OPY2 gene | This study |
5′ UTR L | The 5’UTR of the long transcript Mr-OPY2-L | |
Mr-OPY2-L ΔAUGs | The mutant of Mr-OPY2-Lwith the 5′UTRL uORFs mutated | This study |
Genomic clones | ||
gMr-OPY2 | The genomic clone of the Mr-OPY2 gene | This study |
gMr-OPY2 ΔAUGs | The mutant of gMr-OPY2 with the 5′UTR uORFs mutated | This study |
Strains | ||
WT | The wild-type strain of M. robertsii ARSEF2575 | |
M2880 | A mutant with T-DNA inserted in the ORF of the Mr-OPY2 gene | This study |
ΔMr-OPY2 | The mutant with Mr-OPY2 ORF deleted based on homologous recombination | This study |
C−ΔMr-OPY2 | The complemented strain of ΔMr-OPY2 | This study |
ΔMr-STE50 | The mutant with Mr-STE50 ORF deleted based on homologous recombination | This study |
C-ΔMr-STE50 | The complemented strain of ΔMr-STE50 | This study |
T-Mr-OPY2-LΔ AUGs | The strain with gMr-OPY2Δ AUGs to replace its corresponding region in M. robertsii | This study |
T-Mr-OPY2-L AUG | The strain with gMr-OPY2 to replace its corresponding region in M. robertsii | This study |
ΔMr-OPY2ːːMr-OPY2-S | The strain with Mr-OPY2-S controlled by the constitutive promoter Pgpd | This study |
ΔMr-OPY2ːːMr-OPY2-L | The strain with Mr-OPY2-L controlled by the promoter Pgpd from M. acridum | This study |
ΔMr-OPY2ːː Mr-OPY2-LΔ AUGs | The strain witht Mr-OPY2-LΔ AUGs controlled by the Pgpd from M. acridum | This study |
P404-Mr-OPY2 | The strain with Mr-OPY2 mORF driven by the 404 bp Pgpd from M. acridum | This study |
P683-Mr-OPY2 | The strain with Mr-OPY2 mORF driven by the 683 bp Pgpd from M. acridum | This study |
ΔMero-Fus3 | The deletion mutant of the Fus3-MAPK gene | Ref. 24 |
ΔMero-Hog1 | The deletion mutant of the Hog1-MAPK gene | Ref. 24 |
ΔAftf1 | The deletion mutant of the Aftf1 gene | This study |
C-ΔAftf1 | The complemented strain of ΔAftf1 | This study |
Aftf1 OE | The strain overexpressing the Aftf1 gene | This study |