Table 1 Transcripts and strains in this study

From: Alternative transcription start site selection in Mr-OPY2 controls lifestyle transitions in the fungus Metarhizium robertsii

Name

Description

Ref

Transcripts

Mr-OPY2-S

The short transcript of the Mr-OPY2 gene

This study

5′ UTR S

The 5’UTR of the short transcript Mr-OPY2-S

 

Mr-OPY2-L

The long transcript of the Mr-OPY2 gene

This study

5UTR L

The 5’UTR of the long transcript Mr-OPY2-L

 

Mr-OPY2-L ΔAUGs

The mutant of Mr-OPY2-Lwith the 5′UTRL uORFs mutated

This study

Genomic clones

gMr-OPY2

The genomic clone of the Mr-OPY2 gene

This study

gMr-OPY2 ΔAUGs

The mutant of gMr-OPY2 with the 5′UTR uORFs mutated

This study

Strains

WT

The wild-type strain of M. robertsii ARSEF2575

 

M2880

A mutant with T-DNA inserted in the ORF of the Mr-OPY2 gene

This study

ΔMr-OPY2

The mutant with Mr-OPY2 ORF deleted based on homologous recombination

This study

C−ΔMr-OPY2

The complemented strain of ΔMr-OPY2

This study

ΔMr-STE50

The mutant with Mr-STE50 ORF deleted based on homologous recombination

This study

C-ΔMr-STE50

The complemented strain of ΔMr-STE50

This study

T-Mr-OPY2-LΔ AUGs

The strain with gMr-OPY2Δ AUGs to replace its corresponding region in M. robertsii

This study

T-Mr-OPY2-L AUG

The strain with gMr-OPY2 to replace its corresponding region in M. robertsii

This study

ΔMr-OPY2ːːMr-OPY2-S

The strain with Mr-OPY2-S controlled by the constitutive promoter Pgpd

This study

ΔMr-OPY2ːːMr-OPY2-L

The strain with Mr-OPY2-L controlled by the promoter Pgpd from M. acridum

This study

ΔMr-OPY2ːː Mr-OPY2-LΔ AUGs

The strain witht Mr-OPY2-LΔ AUGs controlled by the Pgpd from M. acridum

This study

P404-Mr-OPY2

The strain with Mr-OPY2 mORF driven by the 404 bp Pgpd from M. acridum

This study

P683-Mr-OPY2

The strain with Mr-OPY2 mORF driven by the 683 bp Pgpd from M. acridum

This study

ΔMero-Fus3

The deletion mutant of the Fus3-MAPK gene

Ref. 24

ΔMero-Hog1

The deletion mutant of the Hog1-MAPK gene

Ref. 24

ΔAftf1

The deletion mutant of the Aftf1 gene

This study

C-ΔAftf1

The complemented strain of ΔAftf1

This study

Aftf1 OE

The strain overexpressing the Aftf1 gene

This study