Table 1 Noninvasive screening of wild-living bonobo communities for Laverania infections

From: Wild bonobos host geographically restricted malaria parasites including a putative new Laverania species

Field sitesa

Conventional cytB screen

Intensified cytB screenb

 
 

Samples testedc

Samples positived

Detection rate (%)

Samples testedc

Samples positived

Detection rate (%)

Combined detection rate (%)

Balanga (BN)

84

0

0

84

0

0

0

Bananjale (BX)

1

0

0

1

0

0

0

Bayandjo (BJ)

2

0

0

2

0

0

0

Ikela (IK)

465

0

0

432e

0

0

0

Kokolopori (KR)

69

0

0

69

1

1.4

1.4f

Lingunda-Boyela (LG)

25

0

0

25

0

0

0

Lomako (LA)

307

0

0

289e

0

0

0

Lui-kotal (LK)

38

0

0

38

0

0

0

Malebo (ML)

262

0

0

n/a

n/a

n/a

n/a

Manzana (MZ)

165

0

0

165

0

0

0

Tshuapa–Lomami–Lualaba (TL2)

138

16

11.6

122

17

13.9

23.9f

  1. n/a not available
  2. aField sites are designated by a two- or three-letter code (their location is shown in Fig. 1). Regular cytB screening data for KR and LK sites have previously been reported2
  3. bSamples initially negative by conventional (diagnostic) cytB PCR were subsequently retested by intensified PCR, performing 8–10 additional amplification reactions per faecal DNA (see Supplementary Table 1 for details)
  4. cThe host species origin of all faecal samples was confirmed by mitochondrial DNA (D-loop) analysis. As previously reported, the number of individuals tested at the KR, LK and TL2 sites was 38, 17 and 63, respectively3
  5. dAll amplification products were sequence confirmed to represent Laverania parasites
  6. eOnly a subset of the originally screened IK and LA samples were available for intensified PCR
  7. fOf a total of 38 and 63 bonobos at the KR and TL2 sites1, 2, 1 and 24 were Laverania infected, indicating a prevalence of 2.6% and 38%, respectively