Fig. 2 | Nature Communications

Fig. 2

From: ER-associated degradation regulates Alzheimer’s amyloid pathology and memory function by modulating γ-secretase activity

Fig. 2

Confirmation of membralin-interacting protein components by FRET and co-IP. a FRET images of cells co-transfected with vectors expressing membralin-mCherry and either SYVN1-EGFP, AMFR-EGFP, EMC3-EGFP or Ubxd8-EGFP. Scale bar, 10 µm. b Quantification of FRET efficiency between membralin and SYVN1, AMFR, EMC3, Ubxd8, Derlin1, EMC1, Ubac2, FAM8A1, respectively. mCherry-7AA-EGFP was used as a positive control (n = 10 cells). Data represent mean ± s.e.m. from three independent experiments. c Co-IP analysis of endogenous membralin interactions with endogenous SYVN1, or AMFR in N2a cells under normal and ER stress conditions induced by tunicamycin (5 μg/ml) or thapsigargin (500 nM) for 4 hrs. The fold effect shown was calculated using the ratio from AMFR or SYVN1 (IP/Input) normalized to the membralin IP (IP/β-actin). Non-treated cell samples were set to 1.0

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