Fig. 6

The Meteor locus controls the cell fate decision between mesendoderm and neuroectoderm. a Genomic deletion in Meteor KO cells. b Expression of Meteor lncRNA in WT and Meteor KO ESCs. Bars represent average of fold change normalized to WT. p value was calculated using a two-tailed t test. (****P < 0.0001). c GFP profile at day 0 and day 3 of differentiation in WT and Meteor KO. Percentage of GFP⁺ cells at day 0 and day 3 in WT and Meteor KO are shown to the right. Bars represent average of GFP + cells. p values were calculated using a two-tailed t test. (****P < 0.0001). d Anti-GFP antibody staining on day 0 colonies and day 3 EBs in WT and Meteor KO (GFP: green; DAPI: blue). Scale bar represents 50 μm. e Expression of Meteor lncRNA, Eomes, T, Mixl1, Gsc, Mesp1 and Myh7 in WT and KO cells during cardiogenic differentiation. Trends represent average of fold change normalized to WT ESC. p values were calculated using a two-way ANOVA test. (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). f Heatmap of expression of representative PCGs in d0-d3 WT and Meteor KO cells. g Volcano plot representation of expressed PCGs in d0 WT and d0 Meteor KO. FPKM values of Lhx1 and Nkx6-3 are shown. Bars represent mean expression ± SEM. p values were calculated using a two-tailed t test. (*P < 0.05; ****P < 0.0001). h TUBB3 staining in neuronal-like day 14 WT and Meteor KO cells. (TUBB3: green; DAPI: blue). Scale bar represents 50 μm. Percentage of the TUBB3 positive area is shown below. Bars represent average of percentage. p value was calculated using a two-tailed t test. Expression of Nestin and Nefm in d0-d14 WT and Meteor KO cells. Bars represent average of fold change normalized to day0 WT. p values were calculated using a two-tailed t test. (**P < 0.01; ***P < 0.001). i H3K27Ac and H3K4me3 enrichment at enhancers and promoters of Nkx6.3, Lhx1, Neurog3 and Eomes in d0-d3 WT and Meteor KO cells. Bars represent average of percentage of input. p values were calculated using a two-tailed t test. (*P < 0.05; ***P < 0.001)