Fig. 3
PLIC quantitative analysis of protein post-translational modification in mTECs. a A graphical scheme illustrating PLIC analysis of acetylated Aire, using mouse anti-Aire #209 antibody, and rabbit anti-lysine acetylation antibody. b PLIC analysis showing representative images of Aire acetylation in mTEChi and CD45+ cells isolated from Sirt1 +/– and Sirt1 –/– mice. Presented as (left to right): bright field (BF), PLA (green), staining of CD80 (PB), EpCAM (APC), CD45 (APC/Cy7), and BF/PLA/EpCAM overlay (merged). Scale bar 7 μM. c Quantitative analysis of Aire acetylation (presented as the percentage of PLA+ cells) in mTEChi or CD45+ cells isolated from Sirt1 +/– and Sirt1 –/– mice. Shown are values from three independent experiments. Each point on the graph (triangle for mTEChi, circle for CD45+, and cross for probes only) represents an average value of single independent experiment, calculated from 2 to 4 biological replicates, +s.e.m; **p-value = 0.0014 (independent student t-test)
