Fig. 1

Morgana is essential for TNBC cell invasion and in vivo metastasis formation. a Immunoblotting of Morgana and Vinculin, as loading control, in MCF7, MDA-MB-231, BT549 and MCF10A cell lines. b Immunoblotting of Morgana and Vinculin in MDA-MB-231 cells infected with empty vector (EMPTY) or two independent Morgana shRNAs (shMORG1, shMORG2). c Growth curves of MDA-MB-231. d Quantification of invasion assays performed on MDA-MB-231 EMPTY or shMORG1 and shMORG2. e Quantification of soft agar colony formation by MDA-MB-231 EMPTY or shMORG1 and shMORG2. f–h Representative haematoxylin and eosin stained sections f, quantification of g pulmonary metastases and h metastatic burden, 4 weeks after tail vein injection of MDA-MB-231 EMPTY or shMORG1 and shMORG2 (n = 6 NSG mice per group). i Metastasis quantification in distant organs in mice injected with MDA-MB-231 EMPTY or shMORG1 and shMORG2 (n = 3 NSG mice per group). j Tumor weight 5 weeks after subcutaneous injection of MDA-MB-231 EMPTY or shMORG1 and shMORG2 (n = 6 NSG mice per group). k–m Representative haematoxylin and eosin stained sections k, quantification of l pulmonary metastases and m metastatic burden of MDA-MB-231 EMPTY or shMORG1 and shMORG2 (n = 6 NSG mice per group). Data are the results of at least three independent experiments. Bars in graphs represent standard errors (*p < 0.05; **p < 0.01; ***p < 0.001)