Fig. 8
From: The IKK/NF-κB signaling pathway requires Morgana to drive breast cancer metastasis
High Morgana levels in cancer cells induce neutrophil recruitment in the lung pre-metastatic niche. a, b Rapresentative haematoxylin and eosin stainings (left), quantification of lung metastatic area (middle) and number of lung metastases (right) of mice, 30 days after subcutaneous injection of 4T1 a and E0771 b EMPTY or shMORG1 and shMORG2 infected or not with IκBα shRNA (n = 3 BALB/c or C57BL/6 mice per group). c Percentage of immune cells detected in lungs of mice, 10 days after injection of 4T1 EMTPY or shMORG1 and shMORG2 cells (n = 3 BALB/c mice per group). d Percentage of neutrophils recruited in the lungs of each mouse 10 days after injection of 4T1. e Percentage of immune cells detected in lungs of mice, 14 days after injection of E0771 EMTPY or shMORG1 and shMORG2 cells (n = 3 C57BL/6 mice per group). f Percentage of neutrophils recruited in the lungs of each mouse 14 days after injection of E0771. g Gene expression analysis by Real-time PCR of neutrophil-attracting chemokines and pre-metastatic niche markers analyzed in lungs of mice 14 days after injection of E0771 EMPTY or shMORG1 and shMORG2 (n = 3 C57BL/6 mice per group). h Spearman correlation in mRNA levels between CHORDC1 (Morgana coding gene) and neutrophils marker (FCGR3A and FCGR3B). Bars in graphs represent standard errors (*p < 0.05; **p < 0.01; ***p < 0.001)
