Fig. 5
Expression of miR-21-5p is increased in antagomir-transfected macrophages exposed to capsaicin-released exosomes. a Peritoneal macrophage intracellular expression of miRs 21-5p, Let7b-5p, 155-5p, and 134-5p normalized to SNORD 202 (housekeeping non-coding RNA; n = 4 cultures). b miR-21-5p expression in macrophages transfected with miR-21-5p antagomir or scrambled oligomer and incubated with or without EVs derived from CAPS-treated pure DRG neurons. c miR-21-5p expression in macrophages transfected with miR-21-5p antagomir or scrambled oligomer and incubated with or without miR-21-overexpressing EVs derived from CAPS-treated DRG neurons. Data are means ± S.E.M., n = 3; ***P < 0.001, compared to scrambled oligomer; # P < 0.05, compared to macrophages not exposed to EVs, one-way ANOVA, post hoc Bonferroni test. d Expression of Nos2 mRNA in macrophages incubated with GFP-overexpressing or miR-21-overexpressing neuronal EVs. Data are means ± S.E.M., n = 3; *P < 0.05 compared to macrophages not exposed to EVs; # P < 0.05 compared to GFP-derived EVs, one-way ANOVA, post hoc Bonferroni test. e Mrc1 and Spry2 mRNA expression levels in macrophages transfected with miR-21-5p antagomir or scrambled oligomer and incubated with miR-21-overexpressing EVs derived from CAPS-treated DRG neurons. Data are means ± S.E.M., n = 3; *P < 0.05 and **P < 0.01 Student’s t-test
