Fig. 1
From: Direction of actin flow dictates integrin LFA-1 orientation during leukocyte migration
Integrins, GFP fusions, and modeling GFP and transition dipole orientation with Rosetta. a Three global conformational states of integrins2. Cartoons depict each integrin domain and GFP with its transition dipole (red double-headed arrows). b Ribbon diagram of the integrin headpiece of αL-T bound to ICAM-1. The GFP insertion site in the β-propeller domain is arrowed. Dipole is shown in red. c Cartoon as in a of ICAM-engaged, extended-open LFA-1 showing direction of leading edge motion and actin flow. Large arrows show pull on integrin-β by actin and resistance by ICAM-1. Axes shown in a, c are similar to those in the reference state in Fig. 6. d Sequences and boundaries used in GFP-LFA-1 fusions. Highlighted residues were completely modeled by Rosetta to link GFP to the integrin (yellow) or altered in sidechain orientation only to minimize energy (orange). e Orientation of the transition dipole in GFP-LFA-1 fusions. Integrin domains are shown as ellipsoids or torus and GFP is shown in cartoon for 1 ensemble member. GFP transition dipoles are shown as cylinders with cones at each end for 20 representative Rosetta ensemble members, with the asymmetry of GFP referenced by using different colors for the ends of transition dipoles (which themselves have dyad symmetry)
