Fig. 6
ADPL quantification of endogenous enzyme activity in cellular co-culture and primary patient samples. a, b Representative ADPL images (a) and quantification (b) of NCEH1 activity in primary ovarian cancer spheroids. Simultaneous CD45 staining (green) marks immune cells present in heterogeneous spheroids. Quantification in b: immune cells (n = 25), cancer cells (n = 155). c, d Representative ADPL images (c) and quantification (d) of NCEH1 activity in cellular co-culture of aggressive SKOV3 ovarian cancer cells and primary immune cells. Quantification in d: immune cells (n = 16), cancer cells (n = 20). e, f Representative ADPL images (e) and quantification (f) of NCEH1 activity in cellular co-culture of non-aggressive OVCAR3 ovarian cancer cells and primary immune cells. Quantification in f: immune cells (n = 23), cancer cells (n = 74). g Normalized ADPL signal of OVCAR3, SKOV3, spheroid cancers cells relative to co-cultured CD45 + immune cells (data in b, d, f). Scale bar: 10 μm. Blue channel: DAPI; red channel: ADPL; green channel: CD45; gray channel: DIC. Each point represents a single-cell fluorescence measurement, center line and whiskers denote the mean and 95% C.I. of the population; unpaired Student’s t test was used for statistical significance. ***P < 0.001; ns not significant, Student’s t test. Data are from four or more technical replicates from independent duplicate biological experiments
