Fig. 7
From: Gαi is required for carvedilol-induced β1 adrenergic receptor β-arrestin biased signaling
Gβγ subunits are not required for carvedilol-induced β1AR-mediated ERK phosphorylation. a Validation of the Gβγ inhibition by T8-βARKct. HEK293 cells with or without transient transfection of T8-βARKct were stimulated with 10 μM LPA for 5 min. T8-βARKct diminished the LPA-induced CREB phosphorylation, a known Gβγ-dependent process, confirming the inhibition of Gβγ subunits by T8-βARKct. b The Gβγ subunits are not required for carvedilol-induced β1AR-mediated ERK phosphorylation. β1AR stable cells with or without T8-βARKct transfection was pretreated with vehicle or 200 ng per ml PTX for 16 h. The cells were then stimulated with 10 μM carvedilol or 10 μM isoproterenol for 5 min. T8-βARKct did not have significant effect on ERK phosphorylation, suggesting that Gβγ subunits were not required. Data represent the mean ± SEM for n independent experiments as marked on the figure. Statistical significance vs. unstimulated cells was assessed using one-way ANOVA with Bonferroni correction
