Fig. 2
From: DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle
sgRNA–Cas9 complexes on DNA. a Map and the corresponding HS-AFM image of Cas9-labeled BRCA1 amplicon. Red tick marks on the map indicate locations of the Alu repeat-targeted sgRNA-binding sites. In the image, sgRNA–Cas9 protein complexes appear as 3-nm-tall bumps on the DNA backbone. The amplicon sequence includes five perfect matches to the 20-bp sgRNA sequence and five single base-pair mismatches. Single base-pair mismatches label at roughly one-half the frequency of perfect matches. The star (*) indicates Cas9 bound at locations with a single mismatch, while all other locations are perfect matches. b, c Cas-9-labeled TERT and HER2 amplicons. The sharp bend induced in the DNA backbone by the sgRNA–Cas9 complex is evident in both examples
