Fig. 3
From: DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle
HS-AFM accurately localizes sgRNA–Cas9 complexes on DNA. a Measured vs. expected position of the Cas9 labels determined for BRCA1, TERT-HER2, and ladder constructs. Error bars represent the 95% confidence interval of the mean. For the TERT-HER2 and ladder data points, the symbol diameter is larger than the 95% confidence interval span. The expected size and sample standard deviation (σ) are indicated at three points for reference. b Sizing closely spaced ladder constructs, end-labeled with Cas9. Vertical red lines inside symbols represent the 95% confidence interval of the mean. See Methods for measurement detail
