Fig. 4
From: DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle
Mapping BCL2–IGH translocations with Cas9. a Maps of PCR amplicons from wild-type BCL2 and IGH with Cas9 labels (different colors for emphasis). The two labels on BCL2 straddle the 300-bp MBR translocation hotspot. b HSAFM images of labeled amplicons. c HSAFM-determined maps of the translocation breakpoints from a follicular lymphoma cell line DOHH2, and the seven patient samples. Identity of the Cas9 labels is determined by measuring their position from the end of the molecule. Maps were confirmed with 10 or more molecules each. In all seven patent samples, the translocation breakpoint mapped to the major breakpoint region (MBR) of BCL2 and the JH region of IGH
