Fig. 6
E1R/R2E, E1R/R4E, and KCNQ1-GFP single-channel recordings. The voltage protocol was the same for all recordings in this figure. The potential was stepped from a holding potential of −80 to +60 mV for 4 s, and then to −40 mV for 0.75 s, as shown above the current traces. a Representative single-channel current recordings made from membrane patches containing a single E1R/R2E channel. b Addition of 50 μM Chromanol 293B silenced all E1R/R2E channel activity after ~3 min of exposure. All-points histogram (blue) of the +60 mV portion of the fourth sweep down in a, with closed (maroon) and open (teal) Gaussian fits. The peak of the open component is 0.022 ± 0.002 pA. c, d Representative recordings of a single E1R/R4E channel (c) or E1R/R4E + KCNE1 (d). All-points histogram (blue) of E1R/R4E of the +60 mV portion of a sweep, showing a significant closed portion, with closed (blue) and open (green) Gaussian fits (c) and of 25 active E1R/R4E + KCNE1 sweeps (d). e Representative traces of single-channel recordings made from membrane patches containing a single GFP-tagged KCNQ1 channel. All points histogram (blue) of the +60 mV portion of sweeps in e, with closed (green) and open (gray) Gaussian fits. The peak of the open component is 0.02 ± 0.004 pA. f Ensemble average of seven active sweeps (of 75 total) of KCNQ1-GFP. The ensemble average was filtered again at 50 Hz to show the current waveform more clearly
