Fig. 5
The gut microbiota of Nlrp3 R258W mice confers resistance to DSS-induced colitis and colorectal cancer. a–e Acute colitis was induced in sh-+/+ (n = 13) and sh-+/R258W (n = 10) mice and 1:1 cohoused (ch-, n = 12 for each genotype) mice. The analyses of body weights (a), colon length (b), disease scores (c), histological examination (d), scale bar is 200 μm, and histological score (e) were conducted as described in Fig. 2, except that the mice were killed on day 7. The data in a, b, c and e are shown as the means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 (one-way ANOVA with Tukey’s post-hoc test). f, g Colon cancer was induced and monitored in singly housed and cohoused mice (n = 6 for each group), the representative colon tumor (f) and total tumor size distribution (g) were shown. h–k Feces from the WT and Nlrp3 R258W mice were transplanted to germ-free WT mice (Gnoto-+/+, n = 4, Gnoto-+/R258W, n = 5), and acute colitis was then induced with 2.5% DSS. The diseases were assayed as described in Fig. 2, except that the mice were killed on day 9. The data in h, j and k are shown as the means ± SEM. *P < 0.05, **P < 0.01 (two-tailed Student’s t test). Survival rate is shown in i, *P < 0.05 (Log-rank (Mantel–Cox) test)
