Fig. 5
Expression of 2′OMe RNA oligonucleotides mimicking Zeb2-NAT. a Position of 2′OMe RNA oligonucleotides relative to Zeb2 RNA. b, c qRT-PCR analysis of Zeb2 transcripts (primers Zeb2 #1 and Zeb2 intron, see Supplementary Table 2) in fibroblasts from old mice transfected as indicated. d Immunoblot for Zeb2 and β-actin in total cell lysates of old fibroblasts transfected as indicated. e Representative reprogramming experiment stained for Alkaline Phosphatase after 3 weeks in culture with doxycycline. f Reprogramming efficiency of old fibroblasts transfected as indicated. For all graphics depicted, Student’s t test (two-tailed) statistics, *p < 0.05. At least three independent experiments were carried per condition
