Fig. 8
From: Parkin targets HIF-1α for ubiquitination and degradation to inhibit breast tumor progression
Low Parkin expression is correlated with increased HIF-1α levels and poor distant metastasis-free survival in human breast cancer. a, b Low Parkin expression was significantly correlated with increased HIF-1α levels in human breast cancer specimens analyzed by IHC staining. Upper panel in a: representative IHC staining images in TMA-RCINJ (n = 200, from the RCINJ). Scale bar: 20 µm. Lower panel in a: summary of IHC staining results of human breast cancer specimens in TMA-RCINJ. b Summary of IHC staining results in two different cohorts of human breast cancer specimens in TMAs (n = 120 in TMA-BR2082a; n = 220 in TMA-BR2281 from US Biomax). c, d Low Parkin expression was significantly associated with poor distant metastasis-free survival in human breast cancer. In c, low Parkin expression was significantly associated with poor distant metastasis-free survival in TMA-RCINJ. In d, the survival information and mRNA levels of Parkin were obtained from GEO databases. In c, d: Kaplan–Meier curves were drawn by GraphPad Prism software. Differences between the two survival curves were analyzed using the log-rank (Mantel–Cox) test. e A model depicting the suppression of cancer metastasis by Parkin-mediated HIF-1α ubiquitination and degradation
