Fig. 1

Normal T cell development in Trpm7 ^R/R mice but altered cytokine secretion. a Total WT or Trpm7 ^R/R cell recovery from thymus. b Representative dot plot analysis of thymocytes from WT or Trpm7 ^R/R thymi stained with CD4 and CD8 mAbs. Percentages are shown in each gate. c Dot charts comparing the total number of thymocytes in the double-negative (DN), double-positive (DP), CD4⁺, and CD8⁺ thymocytes are shown (mean ± s.e.m. n = 5). d Representative dot plot analysis of thymocytes gated on DN cells from WT or Trpm7 ^R/R thymi stained with CD44 and CD25 mAbs. Percentages are shown in each gate. e Representative histogram overlay of cell surface CD25 in WT or Trpm7 ^R/R thymocytes. f Dot charts showing the number of total cells (mean ± s.e.m. n = 5) of DN population found in the DN1, DN2, DN3 and DN4 stages. Data are representative results of two independent experiments with five mice per experiment. g Basal cytokine levels evaluated in serum of WT (black, n = 3–7) and Trpm7 ^R/R (grey, n = 3–7) mice, respectively, and shown as pg ml⁻¹. Bar charts indicate mean ± s.e.m. A total number of seven mice were used for each genotype. Note a significant reduction of serum levels of IL-17 and G-CSF in Trpm7 ^R/R. A two-tailed Student’s t test was used with *p < 0.05; **p < 0.01 and ***p < 0.001