Fig. 2

Selectively reduced intra-epithelial lymphocytes in Trpm7 ^R/R mice. a Dot plot (left) and statistical analyses (right) of intra-epithelial lymphocytes (IEL) from WT or Trpm7 ^R/R mice stained as indicated. Percentages are shown in each gate, bar charts show mean percentages ± s.e.m. (WT, n = 6; Trpm7 ^R/R, n = 7). b Dot plot (left) and statistical analyses (right) of lamina propria lymphocytes (LPL) from WT or Trpm7 ^R/R mice stained as indicated. Percentages are shown in each gate, bar charts show mean percentages ± s.e.m. (n = 7). c Absolute numbers (WT, n = 6; Trpm7 ^R/R, n = 7) of the indicated IELs subsets. Bar charts show mean percentages ± s.e.m. d Absolute numbers (mean ± s.e.m. n = 7) of the indicated LPL subsets. e CD3 immunohistochemical staining of small intestine sections of WT or Trpm7 ^R/R mice and relative quantification (right). Scale bars indicate 100 µm. f Dot blots and statistical analyses of MHCII expression in EpCAM⁺ intestinal epithelial cells (IEC). Percentages are shown in each gate, bar charts show mean percentages ± s.e.m. (n = 3). g Quantitative real-time PCR of T-bet, Foxp3, Rorc and Il-17a expression in purified TCRαβ⁺CD4⁺ IELs from WT or Trpm7 ^R/R mice. h Dot plot and statistical analyses of IFN-γ and IL-17A staining in WT or Trpm7 ^R/R TCRαβ⁺CD4⁺ IELs. Percentages are shown in each gate, bar charts show mean percentages ± s.e.m. (WT, n = 5; Trpm7 ^R/R, n = 8). Data are representative results of at least 3 independent experiments. A two-tailed Student’s t test was used with *p < 0.05; **p < 0.01 and ***p < 0.001