Fig. 1
From: HIF drives lipid deposition and cancer in ccRCC via repression of fatty acid metabolism
Lipid accumulation in ccRCC cells is VHL dependent. a Photomicrographs of RCC4, RCC10, and 786-O renal cell lines with or without VHL stained with Oil Red O 6 days after reaching confluence. b Western blot demonstrating VHL expression in reconstituted lines. β-actin used as loading control. c Quantification of Oil Red O extracted from cells shown in a. d Oil Red O staining of RCC4 cells 2 days after treatment with linoleic/oleic acid (LA/OA). e Quantification of Oil Red O staining of cells in d. f Quantification of Oil Red O staining of RCC4 VHL cells treated with LA/OA cultured in either normoxia (N) or 1% oxygen (H). Statistical tests for all panels were two-tailed Student’s t tests. Scale bar = 10 μm. Error bars represent standard deviations
