Fig. 3
From: HIF drives lipid deposition and cancer in ccRCC via repression of fatty acid metabolism
Lipid deposition in VHL-deficient cells is not due to altered fatty acid import. a Quantification of fluorescence in RCC4 and RCC4 VHL cells at indicated times after exposure to BODIPY-dodecanoic acid. b Quantification of fluorescence in RCC10 and RCC10 VHL cells at indicated times after exposure to BODIPY-dodecanoic acid. c Representative fluorescent images of BODIPY-labeled RCC4 and RCC4 VHL cells at 24 h after exposure to BODIPY-dodecanoic acid. Bar = 10 μm. d Quantitative real-time PCR detection of CD36 expression after shRNA knockdown. e Quantification of Oil Red O staining of RCC4 cells with shGFP or shCD36 knockdown. Error bars represent standard deviations. p values of two-tailed Student’s t tests are displayed
