Fig. 5

MYC regulates neuroendocrine markers and gemcitabine response. a–c Loss of MYC in human PDA cell lines decreases neuroendocrine marker expression. Cells were transfected with siRNA targeting c-MYC or non-targeting control siRNA (siNT), total protein was harvested after 72 h and assessed by Western blot. Fold change in protein expression (relative to GAPDH) in siMYC cells normalized to siNT is shown for three independent experimental replicates. Error bars indicate standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, Student’s two-tailed t-test. d Cells were treated with siNT or siMYC for 24 h, then treated with PBS or gemcitabine for 72 h, and assessed by Western blot. SYP protein expression (normalized to GAPDH) is shown relative to siNT PBS control. gem = gemcitabine. Error bars indicate standard deviation. e Cells were treated with siMYC or siNT for 24 h, and then treated with increasing doses of gemcitabine for 72 h. MTS assay was performed. siNT and siMYC curves are shown relative to PBS control for n = 3 experimental replicates. Error bar indicate standard deviation. *P < 0.05, **P < 0.01, two-way ANOVA