Fig. 6
From: Genome-wide identification and differential analysis of translational initiation
Experimental validations of the computationally predicted smORFs. a rRibo-seq and LTM-based TI-seq RPF count profiles in HEK293 cell line for a predicted uORF in 5′UTR of EIF5. b FLAG-tagged uORF within the context of the host mRNA was ectopically expressed and translation of the predicted polypeptide was detected by western blot with an anti-FLAG antibody. β-actin protein was used as internal control for western blot analysis. c rRibo-seq and LTM-based TI-seq RPF count profiles for a predicted ORF encoded by lncRNA DANCR. d FLAG-tagged smORF within the context of host lncRNA was ectopically expressed and translation of the predicted polypeptide was detected by western blot with an anti-FLAG antibody. e rRibo-seq and LTM-based TI-seq RPF count profiles for a predicted ORF encoded by an intron of BLOC1S3. f FLAG-tagged intronic smORF within the context of the host mRNA was ectopically expressed and translation of predicted polypeptide was detected by western blot with an anti-FLAG antibody
