Fig. 3
From: Ingestion of artificial sweeteners leads to caloric frustration memory in Drosophila
CFM formation requires synaptic activity in DPM neurons, the MB and PAM neurons. a Blocking MB neurons under VT30559-GAL4 control 30 min before, during, and until 2.5 h after l-glucose pre-feeding abolishes the sugar devaluation (F (2,42) = 8.966; p < 0.001; n ≥ 14). b Blocking DPM neurons under VT64246-GAL4 control 30 min before, during and until 1.5 h after l-glucose pre-feeding abolishes the sugar devaluation (F (2,44) = 3.868; p = 0.028; n ≥ 14). c, d DPM neurons respond selectively to sweet stimulation. c Left: imaging protocols; right: time course of response. n ≥ 14. Black bar: stimulus presentation. d Average response to l-glucose, d-glucose and water. DPM neurons responded significantly to l- and d-glucose (t-test, t 12 = 5.290; p < 0.001; for l-glucose and t-test, t 18 = 5.450; p < 0.001 for d-glucose; n ≥ 134) at significantly higher levels than water (F (2,42) = 9.090; p < 0.001; n ≥ 11; p = 0.966 in post hoc comparison between the responses to d- and l-glucose). e Blocking PAM neurons under R58E02-GAL4 control 30 min before, during and until 1.5 h after l-glucose pre-feeding abolishes the sugar devaluation (F (2,25) = 4.066; p = 0.03; n ≥ 9). See also Supplementary Figs. 3, 4. Means are ± SEM; statistical tests: t-test and one-way ANOVA; NS: p ≥ 0.05; *p < 0.05; **p < 0.01 in comparison between two groups for t-test and in post hoc comparisons with both parental controls for ANOVA
