Fig. 4

PAM dopaminergic neurons display lower calcium responses while DPM neurons display a higher calcium response to NAS after pre-feeding. a Pre-feeding protocol used before the imaging experiment: flies were pre-fed or not with l-glucose for 1 min, 24 h before l-glucose stimulation under the microscope. b Time course response of PAM neurons. n = 10. Black bar: stimulus presentation. c Average response to l-glucose. Flies pre-fed with l-glucose displayed a significantly lower response in comparison to non-pre-fed flies (t-test, t ₁₈ = 3.03; p = 0.004; n = 10). d Pre-feeding protocol used before the imaging experiment: flies were pre-fed or not with l-glucose for 1 min, 24 h before l-glucose stimulation under the microscope. e Time course response of DPM neurons projecting on MBs medial lobes. n = 8. Black bar: stimulus presentation. f Average response to l-glucose. Flies pre-fed with l-glucose displayed a significantly higher response in comparison to non-pre-fed flies (t-test, t ₁₄ = 6.2; p < 0.001; n = 8). See also Supplementary Fig. 5. Means are ± SEM; statistical test: t-test; NS: p ≥ 0.05; **p < 0.01; **p < 0.001 in comparison between two groups