Fig. 3
Candidate trisporic acid receptors are class C GPCRs. a Maximum-likelihood phylogeny of seven-transmembrane domains from early-diverging fungi (orange) and five different classes of G-protein-coupled receptors (GPCRs): A (purple), B1 (light blue), B2 (dark blue), F (pink), as well as the fungal-specific class D pheromone receptors (green). Fungal protein identifiers include species and strain designation, as well as GenBank accession number or JGI protein ID. Nonfungal proteins have GPCRdb74 identifiers and UniProtKB75 (SW) accession numbers. Support values are displayed above branches. The GPCR sequence alignment and the complete phylogeny are included in Supplementary Data 3 and Supplementary Data 4, respectively. TAR, candidate trisporic acid receptor. b Expression levels of the candidate tar genes in Rm. Three biological replicates were examined per condition. B(+), endobacteria present; B(−), endobacteria absent; error bars represent s.e.m. c Changes of the candidate tar gene expression patterns during the progression of mating in Rm B(+) mates measured in three replicate cultures per time point. Error bars represent s.e.m.
