Fig. 4

Ras proteins in Mucoromycotina. a Bayesian phylogeny of proteins in the Ras1 and Ras2 ortholog groups. Protein identifiers include species and strain designation, as well as GenBank accession number or JGI protein ID. The Rm ras2 genes DE due to endobacteria and mating are in boldface. Posterior probability values are displayed above branches. The alignment is included in Supplementary Data 5. b Expression levels of ras2-1 in strains of Mucor circinelloides ATCC 1216b sexP and CBS 277.49 sexM measured in mates grown alone before the onset of asexual sporulation and during a mating interaction. Three replicate cultures were examined per condition. Error bars represent s.e.m.. c Expression levels of the ras2-1 gene during mating between Rm ATCC 52813 and ATCC 52811 exhibiting normal mating, restricted mating, and total loss of mating. Each condition had three biological replicates. B(+), endobacteria present; B(−), endobacteria absent; error bars represent s.e.m.