Fig. 4

Fusing an NLS largely rescues the function of full-length but not the C-terminal module of PHYB18. a Confocal images showing the subnuclear localization patterns of PBY18N in 10 μmol m⁻² s⁻¹ R light and BCY18N in red light and darkness. b Box and whisker plots showing the number of small (< 1 μm³) and large (≥1 μm³) photobodies in hypocotyl epidermal cells of PBC and PBY18N seedlings grown under 10 μmol m⁻² s⁻¹ R light. c Images of representative 4-day-old seedlings of the indicated lines grown under 10 μmol m⁻² s⁻¹ R light or in the dark. d Box and whisker plots of hypocotyl measurements of seedlings shown in c. The boxes represent from 25th to 75th percentile; the bars equal to the median values. Samples with different letters exhibit statistically significant differences in hypocotyl length (ANOVA, Tukey’s HSD, P < 0.01, n > 40). e Immunoblots showing the levels of PHYB and PIF3 in 4-day-old Col-0, phyB-9, PBC, PBY18-1, PBY18-2, and PBY18N seedlings grown in 10 μmol m⁻² s⁻¹ R light. The sample of dark-grown pifq was used as a negative control for the PIF3 Immunoblots. RPN6 was used as a loading control. The relative levels of PHYB, PHYB-FP, and PIF3 were normalized against the corresponding levels of RPN6 and are shown below the blots. The asterisks in the PIF3 blots indicate nonspecific bands. f Immunoblots showing the levels of PHYB and PIF3 in 4-day-old Col-0, phyB-9, BCY-2, BCY18, and BCY18N grown either in 10 μmol m⁻² s⁻¹ R light or darkness. Dark-grown pifq samples were used as negative controls for the PIF3 immunoblots. RPN6 was used as a loading control. The relative levels of PHYB and PIF3 were normalized against the corresponding levels of RPN6 and are shown below each blot