Fig. 2

TFS1 but not TFS4 stimulates RNAP transcript cleavage activity. a Outline of transcript cleavage assays. The reactions containing A*/G/UTP mix and the SSV1-T6 promoter fused to a 50 nt C-less cassette were incubated at 75 °C. After 5 min, TFS1 or TFS4 was added to the reaction. Samples were collected at various time intervals (0, 1, 2, 5, 10 and 15 min). b Denaturating PAGE analysis of transcript cleavage products in the presence or absence of TFS1, TFS1-AA or TFS4. c Outline of reactivation assays. The reactions were performed with A*/G/UTP mix using SSV1-T6 promoter fused to 50 nt C-less cassette. After 5 min at 75 °C, TFS1 or TFS4 was added and the reaction was chased with CTP and an excess of ATP. Samples were collected at various time intervals (0, 1, 2, 5, 10 and 15 min). d Denaturating PAGE analysis of reactivation products in the presence or absence of TFS1, TFS1-AA or TFS4