Fig. 6
From: The transcript cleavage factor paralogue TFS4 is a potent RNA polymerase inhibitor
STIV virus infection induces TFS4 expression levels and growth retardation. a Infection time course experiment. The growth curve shows the increase of the optical density of S. solfataricus (strain 2-2-12) following infection by the STIV virus (t = 0). b, c Multiplex Western blot using polyclonal antibodies raised against TFS4 (b), RNAP subunits Rpo4/7 (c) and Alba (loading control) as a function of time following infection by STIV. Immunodetection was performed on two biological replicates. d Growth curves of S. acidocaldarius MW001 harbouring expression vectors encoding TFS1, TFS1-tip4, TFS1-tip4-AAA and LacS (control) under non-inducing or inducing e conditions using sucrose or maltose containing media, respectively. Error bars represent standard deviation from three technical repeats
