Fig. 9
From: Fine control of metal concentrations is necessary for cells to discern zinc from cobalt
Predicted and observed responses to Zn(II). a Calculated fractional occupancy ((P•D + (P•M)•D)/Dtotal) of DNA targets with RcnR (blue line), Zur (red line), FrmRE64H (grey line) or FrmR (black line), or of (P•M)•D/Dtotal (dark red line) for Zn(II)-ZntR, as a function of buffered [Zn(II)], determined using Zn(II) affinities, DNA affinities and abundance values in Table 1. Numbering reflects the order of response visualised for each sensor in Fig. 9b, c. b Representative (n = 3) transcript abundance following 10 min exposure of Salmonella to increasing [Zn(II)] assayed by end point PCR. Arrows identify the lowest observed exogenous [Zn(II)] at which each sensor appeared to respond. Data for control genes are presented in Supplementary Fig. 15 and full gel images in Supplementary Fig. 16. c Transcript abundance for the samples shown in b measured by qPCR (error bars are s.d.). Arrows represent a ≥twofold change in transcript abundance. Heat maps of qPCR data from three biological replicates are presented in Supplementary Fig. 17
