Fig. 2
Peritoneal tissue-resident macrophage mitochondria can be fuelled through abundant peritoneal glutamate. a Bar graph showing the effect of 5× diluted peritoneal fluid (1 mM glucose media lavage followed by cell and protein depletion) in comparison to 1 mM glucose media on mitochondrial parameters in peritoneal tissue-resident macrophages. Data were from one experiment (n = 5–6 separate wells per group) and analysed by paired two-way ANOVA (interaction p < 0.0001) with Sidak’s post-tests. b Heat-map showing the log10 difference from the average metabolite peak areas from gas chromatography-mass spectrometry analysis of the most enriched amino acids in peritoneal lavage fluid and the fatty acids in blood serum, glucose and glutamine act as a reference. Data show five independent samples that were analysed by two-way ANOVA (interaction p < 0.0001) with Sidak’s post-tests. c Stacked graph detailing the addition of glutamine (Q, 0.25 mM) and glutamate (0.5 mM) to peritoneal tissue-resident macrophages and resulting changes in mitochondrial parameters. Data are from the same experiment as a, but increases in mitochondrial function after glutamate and Q represent three independent experiments, data were analysed by two-way ANOVA (interaction p < 0.0001), Sidak’s post-tests are indicated within the bars for parameters vs 1 mM glucose and above representing the synergistic effect of glutamate and Q. All error bars denote mean ± SEM
