Fig. 5

Peritoneal tissue-resident macrophages enhance mitochondrial function during respiratory burst through a glutaminolysis enhancement of complex-II. a Peritoneal tissue-resident macrophage (pTRMØ) and neutrophil basal-OCR. Data show at least 10 wells per group and represents three observations. b Changes in mitochondrial parameters after zymosan (50 μg/ml) to pTRMØ. Data (n = 6 separate wells per group) represent three experiments analysed by paired two-way ANOVA (interaction p < 0.0001) with Sidak’s post-tests. c The maximal mitochondrial capacity from b. d Graph showing the total oxygen consumption of neutrophils after zymosan, ±VAS-2870 (10 μM). Data (9 wells per group from three experiments) were analysed by two-way ANOVA (interaction p = 0.8567), *** the significant effect of VAS-2870 (p = 0.0005). e Oxygen consumption of pTRMØ as in d. Data (6 wells per group from two experiments) were analysed by two-way ANOVA (interaction p = 0.138), * the significant effect of VAS-2870 (p = 0.0331). f A simplified diagram of the electron transport chain (ETC). Inhibitors are shown in red. g The effect of rotenone (200 nM) on total oxygen consumption of neutrophils after zymosan. Data show three wells per group and represents three experiments. h The total oxygen consumption of pTRMØ after addition zymosan in the presence of the ETC inhibitors: oligomycin (1.26 μM), rotenone (200 nM), thenoyltrifluoroacetone (TTFA, 2 mM), antimycin A (1 μM). Data show three wells per group, represents two experiments and were analysed by one-way ANOVA (p < 0.001) with Tukey’s post-tests vs control. i The effect of metabolic drugs f and 2-deoxyglucose(2-DG,100 mM) on the uptake of pHrodo zymosan(50 μg/ml,1 h). Data were analysed by one-way ANOVA (p < 0.001) with Tukey’s post-tests vs control. j Graph showing change in OCR after addition of indicated drugs f and atpenin A5 (1 μM). Post treatment (Post) is 90 min after zymosan, pre-treatment occurs before zymosan (Pre). Data show three wells per group, represents at least two experiments and were analysed by two-way ANOVA (interaction p < 0.0001) with Sidak’s post-tests. k The effect of a 5 h atpenin treatment on OCR in pTRMØ. l Graph showing total oxygen consumption of zymosan-induced pTRMØ after atpenin A5 ±glutamine. Data (6 separate wells per group) is a pool of two experiments and was analysed by two-way ANOVA (Interaction p = 0.0079) with Sidak’s post-tests. All error bars denote mean ± SEM, except c which shows the median, data in a, c, g, k were analysed by Student’s t-test