Fig. 3

Distance-based tree of canonical and Vibrio-type CBM40 sequences. Tree of 51 non-redundant sequences (80% identity level) calculated by neighbour-joining using evolutionary distances estimated by applying the PMB model of amino acid changes, including all sites and using a uniform rate of evolution. The representative sequences corresponding most closely (at least 97% identical) to the 7 bacterial structure-determined sequences are shown with symbols, coloured in accordance with Supplementary Fig. 1: “A”, SpCBM40_NanA; “B”, SpCBM40_NanB; “C”, SpCBM40_NanC; “I”, CpCBM40_NanI; “J”, CpCBM40_NanJ; “R”, RgCBM40; “V”, VcCBM40_NanH. Additionally, “L” denotes MdCBM40_NanL closest to the bacterial sequence of highest identity (70% identical to RgCBM40) as only bacterial sequences were searched