Fig. 5
STD NMR analysis of RgCBM40 binding to sialoglycans. a Reference (top) and difference (bottom) spectra of 3′SL and 6′SL. The strongest signals from the Neu5Ac’s protons are labelled in the difference spectra. b Binding epitope mapping from STD NMR of 3′SL and 6′SL. Legend indicates relative STD intensities normalised at H7: blue, 0–24%; yellow, 25–50%; red 51–100%; larger red dots indicate values over 100%. Sialic acid is the main recognition element. c Binding epitopes mapping from STD NMR of Neu5Gcα2-3Lac and Neu5Gcα2-6Lac. Legend as above. Sialic acid is the main recognition element. The strongest STD intensities from CH2 and the H3s, suggest a reorientation of the Neu5Gc ring in the binding pocket, in comparison to 3′SL and 6′SL
