Fig. 2
From: Loss of the molecular clock in myeloid cells exacerbates T cell-mediated CNS autoimmune disease
Loss of Bmal1 from myeloid cells exacerbates EAE. a Bmal1 Myeloid+/+ or Bmal1 Myeloid−/− mice were immunized to develop experimental autoimmune encephalomyelitis (EAE) with myelin oligodendrocyte glycoprotein (MOG35–55) + complete Freund’s adjuvant (CFA) on d 0, and with either low dose pertussis toxin (PT) (125 ng/mouse) or high dose PT (250 ng/mouse) on d 0 and d 2. Mice were graded for EAE daily. Statistics by Kruskal–Wallis with at least 6 mice per group. b RT-PCR analysis of whole brain d 10 post induction of EAE examining Il1b, Ccl2, Il17a and Ifng expression (n = 9–12). c, d D 3 post immunization draining lymph nodes (LN) were isolated from Bmal1 Myeloid+/+ or Bmal1 Myeloid−/− mice and stained ex vivo for CD3, CD11b, Ly6C and MHCII, gating on live CD3− cells (n = 3–6). Statistics were performed by Mann–Whitney U test (b–d). All data presented as means± standard error of the mean (SEM). RQ relative quantification. *p < 0.05; **p < 0.01; ***p < 0.001
