Fig. 3

Enhanced accumulation of inflammatory CD11c⁺Ly6C⁺ cells producing IL-1β in the CNS of Bmal1 ^{Myeloid−/−} mice during EAE. a–c Bmal1 ^Myeloid+/+ and Bmal1 ^{Myeloid−/−} mice were immunized to develop experimental autoimmune encephalomyelitis (EAE) with myelin oligodendrocyte glycoprotein (MOG_35–55) + complete Freund’s adjuvant (CFA) on d 0 and with pertussis toxin (PT) (125 ng/mouse) on d 0 and d 2 (n = 6). a D 10 post EAE induction live cells infiltrating into the spinal cord were FACS stained for CD45 (n = 6). b Live CD45⁺ cells were examined for Ly6C v CD11b or CCR2 v Ly6C (n = 6). c IL-1β expression was determined in live CD45⁺CD11b⁺Ly6C⁺ cells (n = 6). d 10 d post immunization CD11b and Ly6C populations infiltrating the spinal cord of Bmal1 ^{Myeloid−/−} mice were examined for IL-1β expression by FACS, gating on live CD45⁺ cells (n = 6). Data as means ±standard error of the mean (SEM). Statistics were performed by Mann–Whitney U test a–d. *p < 0.05; **p < 0.01